Miscellaneous Reagents

Miscellaneous ReagentsIn chemical reactions, we know different kinds of reagents are often used. Here are some kinds of reagents and how to make it:1. Benedict (qualitative test glucose)Solution ANa Citrate 86.5 g, 50 g Na2CO3, 400 ml distilled water. Dissolve the salt concentrate, help with heating. Strain and dilute with distilled water to 425 ml.Solution BCuSO4.5H2O 8.65 g, 50 ml of distilled water. Combine first is to dissolve all the salt.Mixing processPour B into A while stirring constantly and then dilute to 500 ml.
2. Fehling (solution for reducing sugars)Solution ACuSO4. 5H2O 34.66 g, 5 ml of concentrated H2SO4, distilled water 100 ml. Circulate concentrated H2SO4 slowly into a beaker containing 100 ml of distilled water while stirring occasionally, then put the salt copper sulfate into it. Once dissolved, dilute to 500 ml, transfer the reagent into the reagent bottle.Solution BKNaC4H4O6.4H2O 173 grams, 50 grams of pure NaOH, distilled water. Prepare 250 ml of distilled water in a 600 ml beaker, NaOH was weighed immediately after the insert in distilled water, then dissolve the salt tartat and dilute the solution until the volume to 500 ml.Mixing processDissolve the second volume of this solution with the same ratio at the time will be used (so fresh).3. Silver Nitrate (AgNO3)AgNO3 8.5 gr. 500 ml distilled water.Enter AgNO3 in 500 ml brown bottle closed, pour in 200 ml distilled water, keep the salt is dissolved. Dilute with the remaining distilled water, cover tightly, and store in a dark place or avoid direct sunlight4. Formaldehyde 10%Dilute 40% formaldehyde (formalin) 25 ml to 75 ml of distilled water5. Kupri Acetate (Barfoed reagent for the reducing monosaccharides)Cu (CH3COO) 2 33 gr5 ml of distilled waterInto a 600 ml beaker, mix salt and acetic acid kupri; stir briefly; then pour a little distilled water while stirring so the mixture A dissolves. Laruan dilute the solution until the volume to 500 ml.
6. Millon (reagent for albumin and phenol)Mercury 10 gr20 ml of concentrated HNO3Distilled waterDissolve mercury into concentrated HNO3. (Use the hood and do the acid room). Once mercury is dissolved, add distilled water as much as 2x the volume of the solution obtained by the fancy; let stand for 12 hours, and filtered.7. Molischa. α-naphthol 5% alcoholicα-naphthol 0.5 gramsEthanol 95% 20 mlDissolve A into B, then pour into the bottle drops.b. α-naphthol 15% (called reagents molisch; for wool)15 g α-naphtholalcohol or chloroform 100 mlOnce mixed and shaken; let stand for a while, and then strain.c. β-naphthol40 g β-naphtholalcohol 360 mlOnce mixed, then shaken, let stand a while, then strain.8. Mayer (reagent for alkaloids with almost all white precipitate formed in a slightly acidic)Solution A1.358 g HgCl260 ml distilled waterSolution BKI 5 gr10 ml distilled waterPour solution A into solution B, dilute with distilled water until the solution volume to 100 ml.9. Seliwanoff0.15 g resorcinol34 ml concentrated HCl68 ml distilled water(Or HCl: distilled water = 1: 2)Dissolve A in B.10. 0.5 M Aluminium Sulphate (500 ml)
 Dissolve 166.6 g of Al 2 (SO4) 3 18H2O (MW = 666.5 g / mol) in deionized water sufficient to make 500 ml solution.11. 0.5 M Copper (II) sulfate (500 ml)Dissolve 62.42 g of CuSO4 • 5H2O (MW = 249.7 g / mol) in deionized water sufficient to make 500 ml solution.12. 6.0 M hydrochloric acid (500 ml)Attention: protector, gloves goggles, and lab coat or apron. Working in a fume hood, concentrated hydrochloric acid fumes are caustic and will burn your nose hair. The water is hot enough when concentrated hydrochloric acid is added to waterAdd 258 ml of concentrated HCl (11.6 M and 36%) for which sufficient deionized water to make 450 ml solution. When the solution cools to room temperature, add distilled water until the volume of 500 ml.13. 0.5 M iron (II) sulfate (500 ml)Dissolve 69.5 g of FeSO4 • 7H2O (MW = 278.0 g / mol) dissolved in distilled water until the volume of 500 ml.14. 2 M Methanol Hydrochloric AcidDissolve 17 ml of concentrated hydrochloric acid in 83 ml of methanol.15. 0.5 M Sodium Hydroxide (1 L): Dissolve 20.00 g NaOH (MW = 40.00 g / mol) in enough deionized water to make 1 L solution.(Caution: Wear protective gloves, goggles, and lab coats. Working in the dense fume. Avoid inhaling dust or fumes.)16. 2% aqueous Potassium Permanganate (KMnO4)
 Dissolve 1 g of potassium permanganate in 50 ml of deionized water.17. Potassium Hydroxide 10% methanolDissolve 10 g potassium hydroxide pellets with a total volume of 100 ml of methanol.18. 0.5% ninhydrin solution (50 ml)Dissolve 0.25 g of ninhydrin in 50 ml of 95% ethanol. Place the solution in dropper bottles for filling.19. 2% pyridinium Bromide Perbromide, PBP, in absolute ethanol (50 ml)Dissolve 1 g perbromide pyridinium bromide in 50 ml of absolute ethanol reagent is unstable, so had to put up a new one every day.20. Barfoed's reagent (300 ml)20.0 g Cu (C2H 3O2) 2 • H2O copper (II) acetate monohydrate (MW = 199.65 g / mol) in 300 ml aquades. Dissolve Copper (II) acetate slowly, strain. If necessary, and add 2.7 ml of glacial acetic acid.