Method of Making Section Preparations

Method of Making Section Preparations
Section method is a method of making preparations by means of fixation (depending on material) plant took longer effective ± 3 days. Section method is a method by means of thin meniris on preparations to be made. In principle there are three kinds of slices by cutting areas, namely:a. Transverse slices (cross sections, usually abbreviated to cs or xs) is sliced ​​with a direction perpendicular to the horizontal axis of the object.b. Sliced ​​lengthwise (longitudinal section, usually abbreviated to ls) adalh slices parallel to the horizontal axis of the object.1. Radial longitudinal slices: if preparations were sliced ​​perpendicular to the axis of the organ.2. Tangential longitudinal slices: if the direction of the cut does not pass through the axis of the organ but only parallel to the axis of the organ. Actually understanding is parallel to the outer surface of a plant.3. For the trunk of the plant such as a square-shaped cross section so that the cat's whiskers sliced ​​lengthwise cross-section through the axis is called the diagonal longitude.4. For materials such as castor beans that have a symmetric field, then the cross-sectional longitudinal cross-sectional slice through the axis is called the median longitude.c. Middle slices (median section, usually abbreviated med.atau m.) is sliced ​​parallel to or perpendicular to the center of an object.Making slice preparation (section preparation) is aimed at objects both large and thick vegetation and animals, so that tissue and the cells can be seen under the microscope, it should be thinned with a road cut into small sections and thin. Some materials such as small twigs, the tip of the stem that is still easy, can be cut or sliced ​​into pieces thin enough to use a razor or razor blade. These materials are not as strong as leaves, roots and tissues of animals in order to cut thin should be supported with a cork, paraffin or other materials.To obtain a sufficiently thin slices in accordance with the desired, use a slicer that can cut tissue micron in size. To be sliced ​​with a microtome then the object or the network must first be infiltrated and blocked with paraffin.Microtome is a machine for slicing of biological specimens into very thin sections for microscopic examination. Some microtome using a steel knife incision and used to prepare animal or plant tissue in histology. Some use of microtome:Some use of microtome:a. For light microscopy, the material first fixed and frozen or embedded in paraffin. Sections as thick as 30-20 mm are usually cut with a steel blade.b. For electron microscopy, fixation followed by embedding in resin as Araldine (R), sections cut with glass knives or diamond knives ultramikrotom thick as 200-100 nm.One type of microtome microtome hand, a hand microtome microtome with the simplest form. These devices are commonly used in school laboratories to make a very thin slice specimens (approximately 20), that can be viewed under a microscope. For example incision leaves, stems, roots, etc.