Method of Making Smear Preparations

Method of Making Smear Preparations
Topical method (smear method) is a way to make preparations by greasing or create a thin membrane of material in the form of liquid or not liquid on glass objects. This method can be used for the manufacture of preparations of blood, pus red bone marrow, oral mucosa, and vaginal mucosa (for knowing a pregnant animal or not), plants sekulen. Here is one example of a method of making preparations commentator smear of blood.Materials used: human blood, 70% ethanol, methanol, gemsa solution of 3% in methanol, cold distilled water previously boiled, entellan. The tools used are glass objects and glass cover, pipettes, needles Franke, tissue, like coloring. Manufacturing process is done by:a. Prepare a clean glass objects in accordance with the desired amount.b. Take the blood from the fingertip to - 3, 4, or 5 with piercing using needles previously Franke either fingertip or a needle was swabbed first with 70% ethanol.c. Discard the first drop of blood out of his fingertips, and use of blood drops the second, third and so on.d. The blood drops on a glass object, then quickly and carefully dab the blood by means of other glass objects. A good basting angle is 45o and when basting quickly in order to obtain a thin smear.e. Let the results of the blood smear to dry in the air, then fiksasilah with methanol for 5 minutes, by immersing the glass objects that have had blood smeared into the container (staining jar) which already contain methanol.f. Let dry preparations in the air once again, by putting a glass object in a standing position like a tilt in staining. After drying on the back sleeping position with the surface containing the spread on the top surface.g. Tetesi entire surface of topical preparations with 3% Giemsa dye solution and let stand for 30-40 minutes or longer.h. Wash with cold distilled wateri. Let dry again topical preparations in the air.j. Tetesi earlier with entellan topical preparations, especially on the smear of blood that is estimated to cells was evident, and immediately after hatching entellan, cover the last with a glass cover.k. Check if at the time of closure there were air bubbles, if there is then remove the bubbles were first by pressing the cover glass with a needle.l. Allow to dry, and so the average can be weighted down with ballast, and the label on the glass object is empty.3. Methods MarserasiMarserasi method is a method of making preparations to separate the fibers from hardwood trees. Making preparations maceration aims to see a picture of the forms of plant cells. The way is by separating the cells making up antarselnya. If you've determined what materials are used in the maceration process is then the material should be boiled with water first until soft and then boiled again with 10% KOH which are compounds useful as maseran pectin which separates the cells.Stalks of plants are always used in the maceration process it because the stems of plants is more varied in cell shape but it is also more easily made when compared with animal cells. Trunk of the plant also has a distinctive shape in the image of its constituent networks. So, this maceration method is more suitable when used in cell or plant tissue when compared with cells or tissues in animals.