Method of Making squash Preparations

Method of Making squash Preparations
 
Squash method is a method of making preparations with the cover glass is pressed on. Example: onion root tip mitosis. The process of making preparations with the method of squash at the root of the onion
a. Preparation phase
1. Perform root growth of onion in a plastic cup containing water for 1 week, by piercing the center of the onion horizontally so that only the roots are touching the water.
2. Cut the roots at the lower end of the evening at 00:00 to 00:15, because at the time that the cells in onion root meristems are actively dividing.
3. Cutting the root length of 1 cm from the tip.
4. Soaking the roots in a bottle filled with a solution ampoule FAA.
5. Close the bottle tightly with plastic ampoules and tied securely.
b. Implementation phase
1. Taking a piece of onion root tip of ampoule bottles by using tweezers.
2. Moving into a watch glass.
3. Adding 70% alcohol and let it soak for 2 minutes.
4. Alcohol 70% smoked paper with suction.
5. Adding 1N HCL solution and soak for 5 minutes.
6. Cut off the tip (the root cap) and placed on glass objects.
7. Drops with a solution of the dye solution acetocarmin seebagai.
8. Chopping with a rusty razor blade and closed with a cover glass.
9. Skipping over the light spirits.
10. Crushing / pressing with your thumb or the tip of a blunt pencil.
11. Observing the preparations under the microscope.